Starch Debranching for Syrup & Brewing Plants
Pullulanase (EC 3.2.1.41) — Debranching Enzyme
For syrup, brewing, ethanol and cyclodextrin plants where branched dextrins hold back conversion: Pullulanase from Bacillus acidopullulyticus debranches starch at α-1,6 linkages, supports increased glucose yield in saccharification, improves fermentability of cereal adjuncts, and enables high-maltose syrup and cyclodextrin production. Activity 1,000–3,000 ASPU/g with optimal pH 4.0–5.5 and 55°C–65°C. Brown liquid supplied in 25 kg drums MOQ. Request a quote with the product, quantity and destination.
Years experience
Countries shipped
9001 certified
Pullulanase Enzyme
1,000 – 3,000 ASPU/g
Applications
Pullulanase cleaves α-1,6 branch points in amylopectin and related polysaccharides, improving downstream amylase efficiency across starch conversion, brewing adjunct treatment, baking shelf-life control, and specialty carbohydrate manufacturing.
Starch Saccharification
Debranches amylopectin at α-1,6 linkages during saccharification, boosting glucose yield by 2–4% over amylase-only processes.
Learn more →High-Maltose Syrup
Prepares branched starch for optimal maltose formation, enabling syrups with >50% maltose content for confectionery and processed food applications.
Brewing Adjunct Processing
Improves fermentability of rice and maize adjuncts by reducing residual dextrins, ensuring consistent attenuation in lighter beer styles.
Baking — Anti-Staling
Modifies starch retrogradation and crumb texture to maintain softness and extend product shelf life.
Bioethanol Optimization
Applied with glucoamylase to achieve near-total starch conversion, maximizing ethanol yield per unit grain.
Cyclodextrin Production
Generates linear glucan chains for CGTase to convert efficiently into cyclodextrins.
Product Specifications
| Enzyme Name | Pullulanase (Debranching Enzyme) (EC 3.2.1.41) |
| Activity | 1,000 – 3,000 ASPU/g |
| Optimal pH | 4.0 – 5.5 |
| Optimal Temperature | 55°C – 65°C |
| Appearance | Brown liquid |
| Source | Bacillus acidopullulyticus |
| Shelf Life | 12 months (refrigerated) |
| Packaging | 25 kg drums / 200 L drums |
| MOQ | 25 kg |
| Certifications | ISO 9001, Food Grade, Non-GMO |
Why Source From Us
Verified Debranching Efficiency
Bulk Pullulanase supplied with defined ASPU/g activity for reproducible debranching and predictable performance in starch conversion lines.
Process-Compatible Conditions
Consistent activity within pH 4.0–5.5 and 55°C–65°C to help maintain yield stability across industrial batches.
Food Grade & Non-GMO Choices
ISO-managed production with optional Food Grade and Non-GMO supply, complete with COA and TDS for procurement documentation.
Industrial Packaging & Reliable Logistics
Stocked in 25 kg and 200 L drums with MOQ 25 kg, ready for continuous supply to syrup, brewing, baking, and ethanol facilities.
Frequently Asked Questions
What does Pullulanase (EC 3.2.1.41) do in starch processing?
It cleaves α-1,6 branch linkages in amylopectin and pullulan, yielding linear chains that amylases convert more efficiently to glucose or maltose.
What are the recommended pH and temperature ranges for Pullulanase?
For best performance, operate at pH 4.0–5.5 and 55°C–65°C.
How should I interpret the activity value (1,000–3,000 ASPU/g)?
The value represents enzyme units per gram. Higher activity offers faster debranching; confirm dosing in your own process trials.
Can Pullulanase improve glucose yield compared with amylase alone?
Yes. Debranching exposes more substrate to amylase, often delivering 2–4% higher glucose output in saccharification operations.
How should I store Pullulanase to maintain activity?
Keep refrigerated and sealed to ensure 12-month shelf life. Avoid repeated temperature fluctuations; follow COA/TDS storage notes.
What should I send for a quote?
Send the product (activity range or grade), the quantity per order or per month, and the destination country. Pullulanase is packed in 25 kg drums or 200 L drums with a 25 kg minimum order; price and lead time are quoted per order. If you want the offer matched to a trial, add your substrate, dry solids and current enzyme program.
Get a Free Sample & Quote
Send your application details and target dosage. We’ll provide a free sample, COA, and competitive quote within 24 hours for your plant testing.
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