Pullulanase for Measurable Process Gains
For saccharification, brewing, bioethanol and syrup plants: a debranching step to maximize starch conversion and glucose or maltose yield. Request a quote with the product, quantity and destination.
Starch plants lose sugar when branched amylopectin resists full hydrolysis, even with strong amylase activity. Pullulanase (EC 3.2.1.41) from Bacillus acidopullulyticus cleaves the alpha-1,6 branch points, so glucoamylase and other saccharifying enzymes can convert more of each batch. Debranching often delivers 2–4% higher glucose output than amylase alone, depending on the application.
The grade works at pH 4.0–5.5 and 55–65 °C, so it is added at the saccharification step after liquefaction. In high-maltose syrup manufacture it supports products above 50% maltose; in brewing it improves the fermentability of rice and corn adjuncts; in bioethanol it is paired with glucoamylase; in cyclodextrin lines it supplies linear glucan chains for CGTase.
We supply manufacturers, distributors and resellers. The product is a brown liquid with a 12-month shelf life when refrigerated, packed in 25 kg drums or 200 L drums, minimum order 25 kg. For a quote, send the product, the quantity and the destination; for a trial offer, add your substrate, dry solids and current enzyme program.
Starch Saccharification Yield Boost
Pullulanase debranches amylopectin to let glucoamylase and alpha-amylase work to completion. Under pH 4.0–5.5 and 55–65 °C, optimized dosing improves glucose yield by 2–4% while reducing residual dextrins that can affect viscosity, filtration, and syrup quality.
High-Maltose Syrup Production
For syrups exceeding 50% maltose, pullulanase creates a more linear glucan profile that downstream enzymes convert cleanly. Producers gain better control of sweetness, viscosity, and consistency, key for confectionery and processed food formulations.
Brewing Adjunct Fermentability
When using rice, corn, or other adjuncts, pullulanase debranches dextrins that limit fermentability. Applied during saccharification, it supports lighter beer styles with improved attenuation, extract efficiency, and predictable wort composition.
Bioethanol and Cyclodextrins
In ethanol production, pairing pullulanase with glucoamylase increases starch conversion and ethanol yield. In cyclodextrin manufacture, it supplies linear glucans for CGTase, boosting throughput and reducing unconverted starch in the final mix.
| Parameter | Value |
| Activity range | 1,000 – 3,000 ASPU/g |
| Optimal pH | 4.0 – 5.5 |
| Optimal temperature | 55°C – 65°C |
| Appearance | Brown liquid |
| Shelf life | 12 months (refrigerated) |
| Packaging | 25 kg drums / 200 L drums |
| Source | Bacillus acidopullulyticus |
| MOQ | 25 kg |
Frequently Asked Questions
How does pullulanase improve starch processing efficiency?
Pullulanase hydrolyses alpha‑1,6 linkages in branched amylopectin, enabling amylases to access and break down more of the starch molecules. This debranching step translates to higher sugar yields, reduced viscosity, and improved filtration, helping plants achieve more consistent and efficient downstream processing.
What are the ideal conditions for pullulanase activity?
The enzyme operates best at pH 4.0–5.5 and 55–65 °C, conditions common to saccharification stages in starch, brewing, syrup, and ethanol production. Dosing depends on substrate, target conversion, and whether pullulanase is combined with other enzymes in the process.
In which industries is pullulanase commonly applied?
It is widely used in starch saccharification, high‑maltose syrup production, brewing adjunct processing, bioethanol manufacture, and cyclodextrin production. The unifying goal is to debranch starch, improve conversion rates, and maximize product yield and quality.
What should procurement teams look for in a pullulanase supplier?
Key factors include verified activity range, pH and temperature compatibility, shelf life, food‑grade or industry certifications, and consistent batch quality. Reliable logistical support and technical guidance for application‑specific dosing are also important for successful integration.
How does viscosity reduction benefit industrial processes?
By breaking down branched dextrins, pullulanase lowers viscosity in syrups and fermentation broths. This improves mixing, heat transfer, and filtration, reduces pump load, and helps maintain stable operating conditions throughout production.
What should a buyer include in a quote request?
The product or activity range, the quantity (per order or per month) and the destination. Packs are 25 kg drums or 200 L drums and the minimum order is 25 kg; price and lead time are quoted per order. Add the substrate and the target (glucose, maltose or fermentability) if you want the offer matched to your line.
Request a Pullulanase Quote for Your Line
Send your application details and target dosage. We’ll provide a free sample, COA, and competitive quote within 24 hours for your plant testing.
Get Quote